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RevMab
5-Hydroxymethylcytosine (5-hmC) DNA Matched Antibody Pair
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777
CHF
CHF 777.00
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REV-31-1111-MK-KI011 SetCHF 777.00
Figure 1: Detection of 5-Hydroxymethylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Hydroxymethylcytosine (clone RM236) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.
| Product Details | |
|---|---|
| Synonyms | 5-hmC |
| Product Type | Set |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity |
This antibody pair detects 5-hydroxymethylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-caC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays. |
| Crossreactivity | All |
| Quantity |
ELISA: |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Kit Contains |
1) Capture Antibody: Anti-5-Hydroxymethylcytosine Rabbit Monoclonal Antibody, Clone RM236, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins. 2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
5-Hydroxymethylcytosine (5hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC). It not only marks active demethylation but also serves as a relatively stable DNA mark that plays distinct epigenetic roles. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off. 5hmC is abundantly expressed, but the levels vary significantly depending on the cell type. The highest levels are found in neuronal cells of the central nervous system.
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